Tecnologie di espressione genica batterica
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I cloni in pool vengono poi inoculati nel topo. I due tipi principali di strategie di trappola del promotore IVET sono (a) la complementazione della mutazione auxotrofica e (b) l'espressione della resistenza agli antibiotici.
Solo i batteri che contengono il marcatore selettivo fuso con un gene trascrizionalmente attivo nell'ospite sono in grado di sopravvivere. Dopo un adeguato periodo di infezione, i batteri che esprimono il marcatore vengono isolati da polmoni, fegato, milza e tessuto mesenterico.
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£27.18
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