Tecnologias de Expressão Génica Bacteriana
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Os clones agrupados são então inoculados no ratinho. Os dois principais tipos de estratégias de armadilha do promotor IVET são (a) a complementação da mutação auxotrófica e (b) a expressão da resistência a antibióticos.
Apenas as bactérias que contêm o marcador seletivo fundido com um gene que é transcricionalmente ativo no hospedeiro são capazes de sobreviver. Após um período de infeção adequado, as bactérias que expressam o marcador são isoladas do pulmão, fígado, baço e tecido mesentérico.
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