Technologies d'expression génique bactérienne
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Les clones regroupés sont ensuite inoculés à la souris. Les deux principaux types de stratégies de piège à promoteur FIVETE sont (a) la complémentation de la mutation auxotrophe et (b) l'expression de la résistance aux antibiotiques.
Seules les bactéries qui contiennent le marqueur sélectif fusionné à un gène transcriptionnellement actif dans l'hôte sont capables de survivre. Après une période d'infection appropriée, les bactéries qui expriment le marqueur sont isolées du poumon, du foie, de la rate et du tissu mésentérique.
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