Strategien für die heterologe Expression von Proteinen und Genaktivität
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Unter heterologer Expression versteht man die Expression eines Gens oder eines Teils eines Gens in einem Wirtsorganismus, der dieses Gen oder Genfragment von Natur aus nicht besitzt. Sie ist die vorherrschende Technik zur Herstellung interessanter Enzyme für Funktionsstudien und industrielle Anwendungen, wobei Escherichia coli aufgrund seines schnellen Wachstums, der kostengünstigen Kultivierung und der einfachen Einführung exogener Gene ein weit verbreiteter heterologer Wirt ist.
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