RP-HPLC simultâneo para a estimativa de teofilina e montelucaste
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A cromatografia líquida é uma técnica de separação que envolve a colocação (injeção) de um pequeno volume de amostra líquida num tubo com partículas porosas (fase estacionária), onde os componentes individuais da amostra são transportados ao longo do tubo (coluna) por um líquido. A HPLC é óptima para a separação de compostos químicos e biológicos não voláteis.
Na RP-HPLC, o tamanho da coluna é o mesmo, mas a sílica é modificada para a tornar não-polar, ligando longas cadeias de hidrocarbonetos à sua superfície - normalmente com 8 ou 18 átomos de carbono. É utilizado um solvente polar, ou seja, uma mistura de água e um álcool.
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£27.18
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£10.58
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