Rilevamento quantitativo della babesiosi equina con tecniche molecolari: Quantificazione della babesiosi equina mediante PCR in tempo reale - un'alternativa al test ELISA
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Il metodo utilizzato è stato l'estrazione del DNA ematico da cavalli seguita dall'amplificazione con primer specifici per i parassiti di Babesia equi. Sono stati eseguiti due tipi di test: nested-PCR e Q-PCR per identificare e quantificare il parassita.
Sono stati analizzati sette cavalli di razza, tre dei quali presentavano livelli elevati di parassita, pur non avendo sintomi clinici della malattia al momento del prelievo, ma ammalandosi mesi dopo. Questo studio apre la prospettiva di utilizzare la tecnica Q-PCR per diagnosticare questa patologia equina.
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£27.18
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