Quantitative detection of equine babesiosis by molecular techniques: Quantification of equine babesiosis by real-time PCR - an alternative to the ELISA test
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The method used was the extraction of blood DNA from horses followed by amplification with specific primers for Babesia equi parasites. Two types of test were carried out: nested-PCR and Q-PCR to identify and quantify the parasite.
Seven horses of the breed were analysed, three of which showed high levels of the parasite, although they had no clinical symptoms of the disease at the time of collection, but fell ill months later. This study opens up the prospect of using the Q-PCR technique to diagnose this equine pathology.
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£27.18
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