The study aim to isolation and identification of aerobic bacteria from diabetic foot ulcers in diabetic patients and investigation the resistance of bacteria to antibiotic agent. P.

aeruginosa showed a varied levels of resistance to; ceftriaxone, cefoxitin and Cefepime with percentage (100%); tobramycine, ciproflaxcin and levofloxacin with percentage of (41. 1%); ticarcllin with clavulalnic acide, piperacllin, ceftazidime,Amikacin with percentage (35.

2%); aztreonam with percentage of (47. 5); meropenem and imipenem with percentage (23.

5 %). B.

cepacia isolates showed high level of resistance for all β-lactam antibiotic classes which included;, ceftriaxone, cefoxitin and Cefepime, with percentage of (100%); ticarcllin with clavulalnic acide ,piperacllin, ceftazidime, tobramycine, ciproflaxcin and levofloxacin with percentage of (87. 5%); aztreonam and amikacine with percentage(62.

5%); meropenem, imipenem and gentiamycin with percentage of (37. 5%).

P. aerginosa isolates were with blaOXA (47.

1%) and (58. 8%) of isolates carry blaCTX-M gene, all isolates of p.

aeruginosa gave negative result of blaIMP and blaKPC geneand PCR analysis showed that the integron gene was (94. 1%).

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