Molekularepidemiologische Studie der Lepra in Fortaleza, Ceará: Molekulare Epidemiologie der Lepra in Fortaleza, Ceará
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Es wurden NHS-Proben von 185 Patienten, 136 Personen ohne Lepra, die das nationale Referenzzentrum für sanitäre Dermatologie Dona Libânia in Fortaleza, Ceará, besuchten, und 121 Studenten des Christus College in Fortaleza analysiert. Hautbiopsien (SB) von 38 Patienten wurden analysiert.
Alle SN-Proben wurden einer Amplifikation der RLEP-Region mittels NESTED PCR unterzogen. Um die genetische Vielfalt von M.
leprae zwischen Individuen zu bewerten, wurden SN-Stämme von 48 RLEP-positiven Patienten anhand von vier VNTR-Loci (Variable Number Tandem Repeat) analysiert. Um die Variabilität des Stammes bei ein und demselben Individuum zu untersuchen, wurden BP- und SN-Proben von 38 positiven Patienten im RLEP-System anhand der 15 VNTR-Loci verglichen.
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£27.18
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