According to popular belief, appealing to the sense of sight is more powerful than appealing to the sense of hearing, which could be the origin of the adage "Seeing is believing. " Technologies that allow researchers to "see into the body" or "see into cells" are crucial for both basic biological processes research and the detection and treatment of disease.

It is ideal for the procedures to be non-invasive, meaning they shouldn't include slicing into the body or separating its components. Thus, in the biomedical sciences, methods for visualising physiological or pathological changes in the body and cells have grown in significance.

Contributing to the regulation of numerous physiological processes and playing critical roles in preserving the healthy development of living bodies, a few significant biological species and their microenvironments manage a complex and sensitive dynamic balance in life systems. Living things can develop a variety of illnesses or even pass away if their homeostasis is disturbed.

Real-time monitoring of these biological species and their microenvironments during various physiological and pathological processes is therefore extremely important. One of the most effective methods for real-time imaging in biological samples is the use of fluorescent probe-based approaches.

Small-molecule fluorescent probes have developed into effective instruments for exploiting light to enhance the field of cell biology research, find novel medications, identify pollutants in the environment, and increase cancer diagnosis. The growth of the fluorescent probe research community—which was tiny in the late 20th century but now comprises over a hundred research groups worldwide—correlates with these uses.

It took scientists with a lot of courage from many different professions to join this expansion. In this book, pyrene, coumarin, and naphthalimide are among the fluorescent dyes that are most frequently employed for fluorescence imaging.

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