Haplodiploidization in durum wheat by androgenesis
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In addition to the effect of genotype, the different results obtained (callus, embryos and plants) were analyzed as a function of donor heel, cold pretreatment and culture medium (C17 and Olsen). The donor heel had a significant effect on embryo and callus induction, with the master strand giving better results than the first heel.
A non-significant effect of cold pretreatment was demonstrated for embryo induction and callus production, but also on regenerant quality. It was also found that a single embryo produced on Olsen medium gave a chlorophyll plant, whereas those produced on C17 medium gave no plant at all; albinism was also absent.
The genotype factor is very important for embryo induction and regeneration. The results show that durum wheat remains a recalcitrant genotype, especially for seedling regeneration.
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£27.18
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