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La crioconservazione è un processo di conservazione in cui il materiale biologico viene sottoposto a temperature bassissime con azoto liquido (-196ºC). Tuttavia, affinché le strutture possano essere recuperate dopo la crioconservazione, è necessario aggiungere sostanze crioprotettive.
Lo scopo di questo studio è stato quello di sviluppare protocolli per la crioconservazione di semi e polline di orchidee brasiliane. Il successo della crioconservazione dei semi di orchidea, dei pollini e dei loro tessuti in azoto liquido richiede l'uso di crioprotettori.
I trattamenti migliori si basano su una combinazione di crioprotettori extra- e intracellulari. .
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