Análise da diversidade genética de isolados de Trichoderma usando marcadores SSR: Estudo da diversidade genética utilizando marcadores moleculares de Trichoderma
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É um agente de biocontrolo agressivo bem conhecido e tem um elevado potencial de reprodução e colonização. Neste estudo, a diversidade genética entre catorze isolados de Trichoderma (TBT- 01 a TBT- 14) foi avaliada utilizando dez marcadores SSR.
Foram colhidas amostras de solo rizosférico de vários locais de Uttar Pradesh, que foram submetidas a diluição em série e plaqueadas num meio seletivo de Trichoderma. As colónias dos isolados foram cultivadas em ágar Batata Dextrose para isolamento de ADN e estudos moleculares.
Foram utilizados dez iniciadores SSR (SSR-01, SSR- 06, SSR- 08, SSR- 10, SSR- 11, SSR-13, SSR- 14, SSR- 15, SSR- 18 e SSR- 20) para a amplificação por PCR do ADN isolado. .
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£27.18
£28.33 -
£10.58
£12.58 -
£16.71
£17.13
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